DNA

Part:BBa_K4342004:Design

Designed by: Keaton Brown   Group: iGEM22_Austin_UTexas   (2022-10-04)


ACIAD2049 Downstream for P. destructans Detector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 630
    Illegal XbaI site found at 550
    Illegal SpeI site found at 228
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 630
    Illegal NheI site found at 1169
    Illegal SpeI site found at 228
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 630
    Illegal BglII site found at 388
    Illegal BglII site found at 886
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 630
    Illegal XbaI site found at 550
    Illegal SpeI site found at 228
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 630
    Illegal XbaI site found at 550
    Illegal SpeI site found at 228
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to ligate to the tdk/kan cassette and the ACIAD2049 Upstream Homology version 2 via bsaI and bsmbI digestions.



Source

Acinetobacter baylyi ADP1


References